Organoid Gut Microbiome
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NEW: Protocol for modeling the repair of intestinal damage by co-culturing mesenchymal stromal/stem cells and intestinal organoids

Kari-Pekka Skarp, Bahar Yetkin-Arik, Suze A. Jansen, Caroline A. Lindemans, Magdalena J. Lorenowicz
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Highlights

Procedures for the coculture of human MSCs and human duodenal epithelial organoids Steps for light microscopy-based readout of MSC effects on organoids exposed to injury Detailed steps for data quantification using OrganoSeg and OrganoAna tools System compatible with downstream analyses using standard cell biology methods Skarp et al., STAR Protocols 7, 104292 March 20, 2026 © 2025 The Author(s). Published by Elsevier Inc. https://doi.org/10.1016/ j.xpro.2025.104292

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